InVitroCYP™ H-class microsomes

InVitroCYP-H-Class-MicrosomesLiver microsomes provide a readily available and well-characterized biological model for use in CYP enzyme inhibition studies. InVitroCYP H-class microsomes are prepared from tissues that are screened and selected for high activity across the panel of relevant cytochrome P450 enzymes, including those CYPs that are traditionally low in activity. This microsome classification provides researchers with enhanced sensitivity on typically low activity CYPs.

 

 CYPSubstrateMetabolitesUnits
AMOCYP2C8: Amodiaquine DecentralizationAmodiaquineN-Desethylamodiaquinepmole/min/mg
BUPCYP2B6: Bupropion hydroxylationBupropionHydroxybupropionpmole/min/mg
COUMCYP2A6: Coumarin 7-hydroxylationCoumarin7-HCG, 7-HCS, 7-HCpmole/min/mg
CZXCYP2E1: Chlorzoxazone 6-hydroxylationChlorzoxazone6-Hydroxychlorzoxazonepmole/min/mg
DEXCYP2D6: Dextromethorphan O-demethylationDextromethorphanDextrorphanpmole/min/mg
ECODMultiple CYPs: 7-Ethoxycoumarin O-deethylation7-Ethoxycoumarin7-HCG, 7-HCS, 7-HCpmole/min/mg
MEPHCYP2C19: S-Mephenytoin 4'-hydroxylationS-Mephenytoin4'-Hydroxymephenytoinpmole/min/mg
MIDCYP3A4: Midazolam 1-hydroxylationMidazolam1-Hydroxymidazolampmole/min/mg
PHENCYP1A2: Phenacetin O-deethylationPhenacetinAcetominophenpmole/min/mg
TESTCYP3A4: Testosterone 6ß-hydroxylationTestosterone6β-Hydroxytestosteronepmole/min/mg
UGTUGT: 7-Hydroxycoumarin glucuronidation7-Hydroxycoumarin7-Hydroxycoumarin glucuronidepmole/min/mg
TOLBCYP2C9: Tolbutamide methyl-hydroxylationTolbutamide4'-Methylhydroxytolbutamidepmole/min/mg