PBMCs

BioreclamationIVT has recently upgraded our cell processing capabilities and have begun to offer a comprehensive portfolio of peripheral blood mononuclear cells (PBMCs) from a variety of animal species. These cells are manufactured with optimized methods to ensure a high quality product with superior viability, purity and functionality.  Utilization of our cell products can yield a faster time-to-market as less time will be spent on sample preparation and leaving our clients to focus on more technically challenging end point assays.

Researchers can receive PBMCs and other fresh material less than 24 hours after collection. These cell isolations can either be shipped fresh overnight for immediate use, or cryopreserved for future scheduled work.

  • PBMCs can be prepared from any of the products listed below
  • Further processing performed, including RBC lysis, washing, and counting
  • Aliquoting by cell count

BioreclamationIVT prospectively collects patient samples that can meet study-specific inclusion and exclusion criteria. All collections are conducted under IRB-approved protocols, and all donors sign informed consent forms where applicable. All samples are fully characterized and come complete with demographic and medical data, as recorded on case report forms at the time of collection.

  • Collected  from a single donor via leukapheresis
  • Highly enriched for MNC
  • Approximately 1.5 - 2.0 x 10^9 (1.5 - 2.0 billion) PBMCs
  • Derived from two liters of whole blood
  • Equivalent to four times the number of cells of a standard buffy coat.
  • Volume of approximately 140 mL
  • Further processing required to remove excess red blood cells
  • Cell count and FACS analysis can be provided
  • Blood/Cells can be stimulated prior to isolation
  • Same day collection and shipment
  • Repeat donors available
  • Collected at our FDA licensed donor center under IRB approved protocols
  • Donor informed consent approved by IRB
  • High-quality cells as a result of optimized procedures and rigorous testing criteria
  • Peripheral whole blood can be venously collected as full units (approximately 500 mL) or in individual vacutainers (up to 50 mL)
  • Blood can be drawn with the anticoagulant of your choice and the use of specific vacutainers can be requested
  • Suitable for FACS analysis, PBMC preparation, further processing to cell subtypes, culture, etc.
  • Collected at our FDA licensed donor center under IRB approved protocols. Viral tested and found to be negative.
  • Collected at our physicians sites under IRB approved protocols
  • Donor informed consent approved by IRB
  • Shipped fresh overnight for delivery the next morning
  • Contained vacutainers system for the collection, cell separation, and transport of PBMC’s
  • Cells isolated using density gradient fluid
  • Approximately 5-10 x 10^6 (5-10 million) PBMCs per tube
  • Available in Sodium Heparin and Sodium Citrate anticoagulant
  • Tubes can be shipped spun or unspun
  • Once isolated, gel barrier allows for easy transport of cells in the tube
  • Cells can be easily poured off for further processing
  • Collected at our FDA licensed donor center under IRB approved protocols
  • Collected at our physicians sites under IRB approved protocols
  • Donor informed consent approved by IRB
  • Processed from a full unit (500 mL) of whole blood
  • Approximately 5 x 10^8 (500 million) PBMCs
  • Volume of approximately 75-100 mL
  • Further processing required to remove excess red blood cells
  • Same day collection and shipment
  • Viral tested and found to be negative
  • Collected at our FDA licensed donor center under IRB approved protocols
  • Donor informed consent approved by IRB

BioreclamationIVT continues to expand its proprietary Independent Review Board (IRB) approved network of specialty physician sites accessing high quality, consented clinical research samples and data for our clients. Samples, such as whole blood, CPTs and PBMCs can be collected by our physician’s sites including:
 

Autoimmune/RheumatologyCardiovascularDermatology
EndocrinologyGastroenterologyNeurology
Oncology/HematologyPulmonologyVirology