Subcellular Fractions

animal-subcellular-fractionsAnimal microsomes, S9 and  cytosol are available from a variety of species.  Microsomes are a valuable tool for studying the metabolism of your compounds and for examining potential drug-drug interactions.  In addition to pooled microsomes and S9 produced from control laboratory animals, BioreclamationIVT offers fully characterized pooled microsomes and S9 from Sprague-Dawley®™ rats that have been induced with a variety of compounds.

 

Animal Subcellular Products
•    Animal Liver Microsomes & S9
•    Induced Sprague-Dawley Rat Microsomes & S9

All microsomes and s9 products are tested by Quality Control to provide researchers with the best product in the market.  They are vialed at an approximate contration of 20 mg/mL. Each preparation must pass set criteria for release.  Microsomes and S9 are tested for protein.  Human microsomes are tested for total P450. Enzyme characterization is performed on all human microsome and S9 products.

 CYPSubstrateMetabolitesUnits
COUMReaction: Coumarin 7-hydroxylationCoumarin7-HCG, 7-HCS, 7-HCpmole/min/mg
CZXReaction: Chlorzoxazone 6-hydroxylationChlorzoxazone6-Hydroxychlorzoxazonepmole/min/mg
DEXReaction: Dextromethorphan O-demethylationDextromethorphanDextrorphanpmole/min/mg
ECODReaction: 7-Ethoxycoumarin O-deethylation7-Ethoxycoumarin7-HCG, 7-HCS, 7-HCpmole/min/mg
MEPHReaction: S-Mephenytoin 4'-hydroxylationReaction: S-Mephenytoin 4'-hydroxylation4'-Hydroxymephenytoinpmole/min/mg
PHENReaction: Phenacetin O-deethylationPhenacetinAcetominophenpmole/min/mg
TESTReaction: Testosterone 6ß-hydroxylationTestosterone6β-Hydroxytestosteronepmole/min/mg
TOLBReaction: Tolbutamide methyl-hydroxylationTolbutamide4'-Methylhydroxytolbutamidepmole/min/mg
UGTUGT: 7-Hydroxycoumarin glucuronidation7-Hydroxycoumarin7-Hydroxycoumarin glucuronidepmole/min/mg
Total P450 is performed on human and animal microsomes along with protein concentration.
ECOD is the only enzyme characterization performed on animal liver S9 plus the protein concentration.